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Brown Lab movies

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Uchida A, Çolakoğlu G, Wang L, Monsma PC & Brown A (2013)
Severing and end-to-end annealing of neurofilaments in neurons
Proceedings of the National Academy of Sciences USA, 110: E2696–E2705
PubMed
| pdf

  • Movement of a chimeric filament (0.5 MB) view movie
  • Movement of a chimeric filament (1.5 MB) view movie
  • Movement of a chimeric filament (0.2 MB) view movie
  • End-to-end annealing of two axonal neurofilaments (8.3 MB) view movie
  • Severing of an axonal neurofilament (6.1 MB) view movie
  • End-to-end annealing of two axonal neurofilaments (3.6 MB) view movie
  • Severing of an axonal neurofilament (3.2 MB) view movie

Taylor NJ, Wang L & Brown A (2012)
Neurofilaments are flexible polymers that often fold and unfold but they move in a fully extended configuration
Cytoskeleton, 69: 535-544
PubMed | pdf

  • A neurofilament trapped at a branch point (0.9 MB) view movie
  • A folding neurofilament (1.9 MB) view movie
  • A moving neurofilament (13.8 MB) view movie
  • Moving and folding neurofilaments (example 1) (19.2 MB) view movie
  • Moving and folding neurofilaments (example 2) (6.1 MB) view movie
  • Moving and folding neurofilaments (example 3) (3.1 MB) view movie
  • Moving and folding neurofilaments (example 4) (1.8 MB) view movie

Wang L & Brown A (2010)
A hereditary spastic paraplegia mutation in kinesin-1A/KIF5A disrupts neurofilament transport
Molecular Neurodegeneration, 5: 52
PubMed | pdf

  • Neurofilament movement in a cortical neuron (5.9 MB) view movie
  • Neurofilament movement in a cortical neuron (17.7 MB) view movie
  • Neurofilament movement in a cortical neuron (17.1 MB) view movie
  • Neurofilament movement in a cortical neuron expressing N256S-kinesin-1A (8.6 MB) view movie
  • Neurofilament movement in a cortical neuron expressing wild type kinesin-1A (6.8 MB) view movie
  • Microtubule plus-end “comets” in a cortical neuron expressing YFP-EB1 (3.6 MB) view movie

Uchida A, Alami NH & Brown A (2009)
Tight functional coupling of kinesin-1A and dynein motors in the bidirectional transport of neurofilaments
Molecular Biology of the Cell, 20: 4997–5006
PubMed | pdf

  • Neurofilament movement in a wild type neuron (6.2 MB) view movie
  • Low frequency of neurofilament movement in a kinesin-1A knockout neuron (5.6 MB) view movie
  • An anterogradely moving neurofilament in a kinesin-1A knockout neuron (0.3 MB) view movie
  • A retrogradely moving neurofilament in a kinesin-1A knockout neuron (1 MB) view movie
  • Kinesin-1A knockout neuron rescued by expressing kinesin-1A (5.3 MB) view movie

Colakoglu G & Brown A (2009)
Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing
Journal of Cell Biology, 185: 769-777
PubMed | pdf

  • Chimeric neurofilament formed by end-to-end annealing (0.5 MB) view movie
  • Chimeric neurofilament formed by end-to-end annealing (0.4 MB) view movie
  • Circular neurofilament formed by end-to-end annealing (0.5 MB) view movie

Alami NH, Jung P & Brown A (2009)
Myosin Va increases the efficiency of neurofilament transport by decreasing the duration of long-term pauses
Journal of Neuroscience, 29: 6625-6634
PubMed | pdf

  • Movement of a neurofilament in the absence of myosin Va (0.2 MB) view movie

Trivedi N, Jung P & Brown (2007)
Neurofilaments switch between distinct mobile and stationary states during their transport along axons
Journal of Neuroscience, 27: 507-516
PubMed | pdf

  • Pulse escape movie, 5 second intervals (5.3 MB) view movie
  • Pulse escape movie, 5 minute intervals (3.3 MB)  view movie
  • Flash™ animation of fluorescence photobleaching strategy (0.2 MB) view movie
  • Flash™ animation of fluorescence photoactivation strategy (0.1 MB) view movie

Brown A, Wang L & Jung P (2005)
Stochastic Simulation of Neurofilament Transport in Axons: The “Stop and Go” Hypothesis
Molecular Biology of the Cell, 16: 4243-4255
PubMed | pdf

  • Simulation of the movement of pulse of radiolabeled neurofilaments (2.6 MB) view movie
  • Simulation of the movement of neurofilaments in mouse spinal motor axons (4.6 MB) view movie
  • Graphic representation of the simulated moving and pausing behavior (1.3 MB) view movie

Yan Y & Brown A (2005)
Neurofilament polymer transport in axons
Journal of Neuroscience 25: 7014-7021
PubMed | pdf

  • Capture of a neurofilament moving through a naturally occuring gap (2 MB) view movie

Uchida A & Brown A (2004)
Arrival, reversal and departure of neurofilaments at the tips of growing axons
Molecular Biology of the Cell 15: 4215-4225
PubMed | pdf

  • Movement of a neurofilament into a growth cone (0.7 MB) view movie
  • Movement of a neurofilament into a growth cone (5.9 MB) view movie
  • Departure of a neurofilament from a growth cone (0.6 MB) view movie
  • Reversal of a neurofilament in a growth cone (2 MB) view movie
  • Reversal of a neurofilament in a growth cone (0.8 MB) view movie
  • Flash™ animation: hypothesis for neurofilament fate at axon tips (1.2 MB) view movie

Wang L & Brown A (2002)
Rapid movement of microtubules in axons
Current Biology 12: 1496-1501
PubMed | pdf

  • Movement of a microtubule in a photobleached axon (1.6 MB) view movie
  • Movement of a microtubule in a photobleached axon (1.5 MB) view movie

Wang L & Brown A (2001)
Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching
Molecular Biology of the Cell 12: 3257-3267
PubMed | pdf

  • Shorter neurofilament moving anterogradely (1.3 MB)    view movie
  • Longer neurofilament moving anterogradely (4.7 MB) view movie
  • Neurofilament folding up on itself (4.5 MB) view movie
  • Punctate structure moving retrogradely (4.2 MB) view movie

Wang L, Ho C-L, Sun D, Liem RKH & Brown A (2000)
Rapid movement of axonal neurofilaments interrupted by prolonged pauses
Nature Cell Biology, 2: 137-141
PubMed | pdf | News and Views article

  • Several neurofilaments moving anterogradely (2.6 MB) view movie
  • Fast neurofilament passing pausing neurofilament (1.4 MB) view movie