Anthony Brown Lab

Uchida A, Peng J & Brown A (2023)
Regulation of neurofilament length and transport by a dynamic cycle of phospho-dependent polymer severing and annealing
Molecular Biology of the Cell, in press

• Long-range multi-field tracking of a neurofilament (6.5 MB) view movie
• An annealing event (12.3 MB) view movie
• A severing event (8.2 MB) view movie
• Rapid persistent movement of a short neurofilament (5.3 MB) view movie
• Frequent pausing and reversal of a long neurofilament (25.9 MB) view movie
• Movement of neurofilaments containing phospho-deficient mutant NFL (14.6 MB) view movie
• Movement of neurofilaments containing wild type NFL (8.3 MB) view movie
• Movement of neurofilaments containing phospho-mimetic mutant NFL (23.4 MB) view movie
• Movement of neurofilaments containing wild type NFL in neurons treated with cAMP and okadaic acid (14.5 MB) view movie
• Movement of neurofilaments containing phospho-deficient mutant NFL in neurons treated with cAMP and okadaic acid (8 MB) view movie

Fenn JD, Li Y, Julien J-P, Jung P & Brown A (2023)
The mobility of neurofilaments in mature myelinated axons of adult mice
eNeuro 10:ENEURO.0029-23.2023

• A pulse-escape experiment (0.4 MB) view movie
• Neurofilament transport is blocked by glycolytic inhibitors (0.3 MB) view movie

Boyer NP, Julien J-P, Jung P & Brown A (2022)
Neurofilament transport is bidirectional in vivo.
eNeuro 9:ENEURO.0138-22.2022

• Pulse-spread in 2-week-old male tibial nerve (0.4 MB) view movie
• Pulse-spread in 4-week-old male tibial nerve (0.6 MB) view movie
• Pulse-spread in 8-week-old male tibial nerve (0.8 MB) view movie
• Pulse-spread in 16-week-old male tibial nerve (0.7 MB) view movie
• Pulse-spread in 8-week-old female tibial nerve (0.4 MB) view movie
• Pulse-spread in 8-week-old male sciatic nerve (1.4 MB) view movie
• Pulse-spread in 8-week-old male tibial nerve with glycolytic inhibition (0.4 MB) view movie

Ciocanel MV, Jung P & Brown A (2020)
A mechanism for neurofilament transport acceleration through nodes of Ranvier
Molecular Biology of the Cell 31:640-654
PubMed | pdf

• Simulation of the neurofilament content across a node at steady state (0.5 MB) view movie

Fenn JD, Monsma PC & Brown A (2018)
Axonal neurofilaments exhibit frequent and complex folding behaviors
Cytoskeleton 75:258-280
PubMed | pdf

• A neurofilament folding in real time (14.8 MB) view movie
• Animation of the formation of a double hairpin fold (3.3 MB) view movie
• Animation of a filament translocating through a pinch fold (1.4 MB) view movie
• Animation of the formation of a hairpin fold (1.8 MB) view movie
• Animation of the end-to-end annealing of two filaments (6.3 MB) view movie
• Animation of a filament that folds and then moves while folded (1.8 MB) view movie
• Animation of a filament that flips its orientation (2.1 MB) view movie
• Animation of a filament that flips its orientation and reverses direction (3.7 MB) view movie
• Animation of a filament that flips its orientation and then continues to move in the same direction (0.9 MB) view movie
• Animation of a filament that moves while folded and then severs (7.5 MB) view movie

Fenn JD, Johnson CM, Peng J, Jung P & Brown A (2018)
Kymograph analysis with high temporal resolution reveals new features of neurofilament transport kinetics
Cytoskeleton 75:22-41
PubMed | pdf

• Time-lapse movie of EB3-mCherry comets in a cultured cortical neuron (6.7 MB) view movie
• Down-sampled movie of neurofilament movement in a cultured cortical neuron (10.4 MB) view movie
• Real-time movie of neurofilament movement in a cultured cortical neuron (17.6 MB) view movie

Uchida A, Çolakoğlu G, Wang L, Monsma PC & Brown A (2013)
Severing and end-to-end annealing of neurofilaments in neurons
Proceedings of the National Academy of Sciences USA 110:E2696–E2705
PubMed | pdf

• Movement of a chimeric filament (0.5 MB) view movie
• Movement of a chimeric filament (1.5 MB) view movie
• Movement of a chimeric filament (0.2 MB) view movie
• End-to-end annealing of two axonal neurofilaments (8.3 MB) view movie
• Severing of an axonal neurofilament (6.1 MB) view movie
• End-to-end annealing of two axonal neurofilaments (3.6 MB) view movie
• Severing of an axonal neurofilament (3.2 MB) view movie

Taylor NJ, Wang L & Brown A (2012)
Neurofilaments are flexible polymers that often fold and unfold but they move in a fully extended configuration
Cytoskeleton 69:535-544
PubMed | pdf

• A neurofilament trapped at a branch point (0.4 MB) view movie
• A folding neurofilament (0.9 MB) view movie
• A moving neurofilament (8.2 MB) view movie
• Moving and folding neurofilaments (example 1) (10 MB) view movie
• Moving and folding neurofilaments (example 2) (3.5 MB) view movie
• Moving and folding neurofilaments (example 3) (1.7 MB) view movie
• Moving and folding neurofilaments (example 4) (0.9 MB) view movie

Wang L & Brown A (2010)
A hereditary spastic paraplegia mutation in kinesin-1A/KIF5A disrupts neurofilament transport
Molecular Neurodegeneration 5:52
PubMed | pdf

• Neurofilament movement in a cortical neuron (5.9 MB) view movie
• Neurofilament movement in a cortical neuron (17.7 MB) view movie
• Neurofilament movement in a cortical neuron (17.1 MB) view movie
• Neurofilament movement in a cortical neuron expressing N256S-kinesin-1A (8.6 MB) view movie
• Neurofilament movement in a cortical neuron expressing wild type kinesin-1A (6.8 MB) view movie
• Microtubule plus-end “comets” in a cortical neuron expressing YFP-EB1 (3.6 MB) view movie

Uchida A, Alami NH & Brown A (2009)
Tight functional coupling of kinesin-1A and dynein motors in the bidirectional transport of neurofilaments
Molecular Biology of the Cell 20:4997–5006
PubMed | pdf

• Neurofilament movement in a wild type neuron (6.2 MB) view movie
• Low frequency of neurofilament movement in a kinesin-1A knockout neuron (5.6 MB) view movie
• An anterogradely moving neurofilament in a kinesin-1A knockout neuron (0.3 MB) view movie
• A retrogradely moving neurofilament in a kinesin-1A knockout neuron (1 MB) view movie
• Kinesin-1A knockout neuron rescued by expressing kinesin-1A (5.3 MB) view movie

Colakoglu G & Brown A (2009)
Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing
Journal of Cell Biology 185:769-777
PubMed | pdf

• Chimeric neurofilament formed by end-to-end annealing (0.1 MB) view movie
• Chimeric neurofilament formed by end-to-end annealing (0.1 MB) view movie
• Circular neurofilament formed by end-to-end annealing (0.1 MB) view movie

Alami NH, Jung P & Brown A (2009)
Myosin Va increases the efficiency of neurofilament transport by decreasing the duration of long-term pauses
Journal of Neuroscience 29:6625-6634
PubMed | pdf

• Movement of a neurofilament in the absence of myosin Va (0.1 MB) view movie

Trivedi N, Jung P & Brown (2007)
Neurofilaments switch between distinct mobile and stationary states during their transport along axons
Journal of Neuroscience 27:507-516
PubMed | pdf

• Pulse escape movie, 5 second intervals (2.5 MB) view movie
• Pulse escape movie, 5 minute intervals (1.6 MB)  view movie
• Flash™ animation of fluorescence photobleaching strategy (0.2 MB) view movie
• Flash™ animation of fluorescence photoactivation strategy (0.1 MB) view movie

Brown A, Wang L & Jung P (2005)
Stochastic Simulation of Neurofilament Transport in Axons: The “Stop and Go” Hypothesis
Molecular Biology of the Cell 16:4243-4255
PubMed | pdf

• Simulation of the movement of pulse of radiolabeled neurofilaments (0.2 MB) view movie
• Simulation of the movement of neurofilaments in mouse spinal motor axons (0.3 MB) view movie
• Graphic representation of the simulated moving and pausing behavior (0.8 MB) view movie

Yan Y & Brown A (2005)
Neurofilament polymer transport in axons
Journal of Neuroscience 25:7014-7021
PubMed | pdf

• Capture of a neurofilament moving through a naturally occuring gap (0.8 MB) view movie

Uchida A & Brown A (2004)
Arrival, reversal and departure of neurofilaments at the tips of growing axons
Molecular Biology of the Cell 15:4215-4225
PubMed | pdf

• Movement of a neurofilament into a growth cone (0.7 MB) view movie
• Movement of a neurofilament into a growth cone (5.9 MB) view movie
• Departure of a neurofilament from a growth cone (0.6 MB) view movie
• Reversal of a neurofilament in a growth cone (2 MB) view movie
• Reversal of a neurofilament in a growth cone (0.8 MB) view movie
• Flash™ animation: hypothesis for neurofilament fate at axon tips (1.2 MB) view movie

Wang L & Brown A (2002)
Rapid movement of microtubules in axons
Current Biology 12:1496-1501
PubMed | pdf

• Movement of a microtubule in a photobleached axon (1 MB) view movie
• Movement of a microtubule in a photobleached axon (1 MB) view movie

Wang L & Brown A (2001)
Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching
Molecular Biology of the Cell 12:3257-3267
PubMed | pdf

• Shorter neurofilament moving anterogradely (1.3 MB) view movie
• Longer neurofilament moving anterogradely (4.7 MB) view movie
• Neurofilament folding up on itself (4.5 MB) view movie
• Punctate structure moving retrogradely (4.2 MB) view movie

Wang L, Ho C-L, Sun D, Liem RKH & Brown A (2000)
Rapid movement of axonal neurofilaments interrupted by prolonged pauses
Nature Cell Biology 2:137-141
PubMed | pdf | News and Views article

• Several neurofilaments moving anterogradely (2.6 MB) view movie
• Fast neurofilament passing pausing neurofilament (1.4 MB) view movie