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Publications

Nowier RM, Johnson CM, Fenn JD, Brown A & Jung P (2026)
Neurofilament transport kinetics can be explained by the activity of small numbers of independent kinesin and dynein motors
Biophysical Journal, in press
PubMed


Jahan N, York HM, Bailey HM, Swann IC, Leterrier C, Spiliotis ET, Aiken J, Tymanskyj SR, Brown A, Winckler B & Fu MM (2026)
Integrating cytoskeletal systems in neurons and glia: dynamic orchestration of structure and function
Journal of Neuroscience, in press


Uchida A, Peng J & Brown A (2023)
Regulation of neurofilament length and transport by a dynamic cycle of phospho-dependent polymer severing and annealing
Molecular Biology of the Cell 34:ar68, 1–15
PubMed | pdf | movies


Fenn JD, Li Y, Julien J-P, Jung P & Brown A (2023)
The mobility of neurofilaments in mature myelinated axons of adult mice
eNeuro
 10:ENEURO.0029-23.2023
PubMed | pdf | movies


Nowier RM, Friedman A, Brown A & Jung P (2023)
The role of neurofilament transport in the radial growth of myelinated axons
Molecular Biology of the Cell
 34:ar58, 1–14
PubMed | pdf | MBoC Science Sketch video


Boyer NP, Julien J-P, Jung P & Brown A (2022)
Neurofilament transport is bidirectional in vivo
eNeuro
 9:ENEURO.0138-22.2022
PubMed | pdf | movies


Stone EJ, Kolb SJ & Brown A (2021)
A review and analysis of the clinical literature on Charcot-Marie-Tooth disease caused by mutations in neurofilament protein L
Cytoskeleton 78:97-110
PubMed | pdf


Boyer NP, Azcorra M, Jung P & Brown A (2020)
Imaging and analysis of neurofilament transport in excised mouse tibial nerve
Journal of Visualized Experiments 162:e61264
PubMed | video | materials


Johnson C, Fenn JD, Brown A & Jung P (2020)
Dynamic catch-bonding generates the large stall forces of cytoplasmic dynein
Physical Biology 17:046004
PubMed | pdf


Ciocanel MV, Jung P & Brown A (2020)
A mechanism for neurofilament transport acceleration through nodes of Ranvier
Molecular Biology of the Cell 31:640-654
PubMed | pdf | movie


Stone EJ, Uchida A & Brown A (2019)
Charcot-Marie-Tooth disease type 2E/1F mutant neurofilament proteins assemble into neurofilaments
Cytoskeleton 76:423-439
PubMed | pdf


Walker CL, Uchida A, Li Y, Trivedi N, Fenn JD, Monsma PC, Lariviere RC, Julien J-P, Jung P & Brown A (2019)
Local acceleration of neurofilament transport at nodes of Ranvier
Journal of Neuroscience 39:663-677
PubMed | pdf


Lai X, Brown A & Xue C (2018)
A stochastic model that explains axonal organelle pileups induced by a reduction of molecular motors
Journal of the Royal Society Interface 15:20180430
PubMed | pdf


Fenn JD, Monsma PC & Brown A (2018)
Axonal neurofilaments exhibit frequent and complex folding behaviors
Cytoskeleton 75:258-280
PubMed | pdf | movies


Fenn JD, Johnson CM, Peng J, Jung P & Brown A (2018)
Kymograph analysis with high temporal resolution reveals new features of neurofilament transport kinetics
Cytoskeleton 75:22-41
PubMed | pdf | movies


Baas PW, Bamburg JR, Brown A & Gonzalez-Billault C (2016)
An EMBO Workshop on Emerging Concepts of the Neuronal Cytoskeleton: a unique venue to discuss recent advances in cellular and molecular aspects of cytoskeleton function in nerve cells
Cytoskeleton 73:422-423
PubMed | pdf


Uchida A, Monsma PC, Fenn JD & Brown A (2016)
Live-cell imaging of neurofilament transport in cultured neurons
Methods in Cell Biology 131:21-90
PubMed | pdf


Green JA, Schmid CL, Bley E, Monsma PC, Brown A, Bohn LM & Mykytyn K (2015)
Recruitment of β-arrestin into neuronal cilia modulates somatostatin receptor subtype 3 ciliary localization
Molecular and Cellular Biology 36:223-235
PubMed


Johnson C, Holmes W, Brown A & Jung P (2015)
Minimizing the caliber of myelinated axons by means of nodal constrictions
Journal of Neurophysiology 114:1874-1884
PubMed | pdf


Xue C, Shtylla B & Brown A (2015)
A stochastic multiscale model that explains the segregation of axonal microtubules and neurofilaments in neurological diseases
PLoS Computational Biology 11:e1004406
PubMed | pdf | Supporting information


Brown A (2014)
Slow axonal transport
In “Reference Module in Biomedical Sciences”, ed. M Caplan, Elsevier
pdf


Koemeter-Cox A, Sherwood TW, Green JA, Steiner RA, Berbari NF, Yoder BK, Kauffman AS, Monsma PC, Brown A, Askwith CC & Mykytyn K (2014)
Primary cilia enhance kisspeptin receptor signaling on gonadotropin-releasing hormone neurons
Proceedings of the National Academy of Sciences USA 111:10335-10340
PubMed | pdf


Li Y, Brown A & Jung P (2014)
Deciphering the axonal transport kinetics of neurofilaments using the fluorescence photoactivation pulse-escape method
Physical Biology 11:026001
PubMed | pdf


Monsma PC, Li Y, Fenn JD, Jung P & Brown A (2014)
Local regulation of neurofilament transport by myelinating cells
Journal of Neuroscience 34:2979-88
PubMed | pdf


Uchida A, Çolakoğlu G, Wang L, Monsma PC & Brown A (2013)
Severing and end-to-end annealing of neurofilaments in neurons
Proceedings of the National Academy of Sciences USA 110:E2696–E2705
PubMed | pdf | movies


Brown A (2013)
Axonal transport
In “Neuroscience in the 21st century: from basic to clinical”, 1st edition, ed. DW Pfaff, Springer, New York, pp. 255-308
pdf


Brown A & Jung P (2013)
A critical reevaluation of the stationary axonal cytoskeleton hypothesis
Cytoskeleton 70:1-11
PubMed | pdf


Monsma PC & Brown A (2012)
FluoroMyelin™ Red is a bright, photostable and non-toxic fluorescent stain for live imaging of myelin
Journal of Neuroscience Methods 209:344-350
PubMed | pdf


Taylor NJ, Wang L & Brown A (2012)
Neurofilaments are flexible polymers that often fold and unfold but they move in a fully extended configuration
Cytoskeleton 69:535-544
PubMed | pdf | movies


Li Y, Jung P & Brown A (2012)
Axonal transport of neurofilaments: a single population of intermittently moving polymers
Journal of Neuroscience 32:746-758
PubMed | pdf | Featured in This week in the Journal


Yuan L, Zheng YF, Zhu J, Wang L & Brown A (2012)
Object tracking with particle filtering in fluorescence microscopy images: application to the motion of neurofilaments in axons
IEEE Transactions on Medical Imaging 31:117-130
PubMed | pdf


Wang L & Brown A (2010)
A hereditary spastic paraplegia mutation in kinesin-1A/KIF5A disrupts neurofilament transport
Molecular Neurodegeneration 5:52
PubMed | pdf | movies


Uchida A, Alami NH & Brown A (2009)
Tight functional coupling of kinesin-1A and dynein motors in the bidirectional transport of neurofilaments
Molecular Biology of the Cell 20:4997–5006
PubMed | pdf | movies | Featured in MBC Incytes


Jung P & Brown A (2009)
Modeling the slowing of neurofilament transport along mouse sciatic nerve
Physical Biology 6:046002
PubMed | pdf


Çolakoğlu G & Brown A (2009)
Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing
Journal of Cell Biology 185:769-777
PubMed | pdf | movies | Featured in Nature Reviews Research Highlights


Alami NH, Jung P & Brown A (2009)
Myosin Va increases the efficiency of neurofilament transport by decreasing the duration of long-term pauses
Journal of Neuroscience 29:6625-6634
PubMed | pdf | movie


Brown A (2009)
Slow axonal transport
In “New Encyclopedia of Neuroscience”, ed. LR Squire, Academic Press, Oxford, Vol. 9, pp. 1-9
pdf


Yan Y, Jensen K & Brown A (2007)
The polypeptide composition of moving and stationary neurofilaments
Cell Motility and the Cytoskeleton 64:299-309
PubMed | pdf


Trivedi N, Jung P & Brown A (2007)
Neurofilaments switch between distinct mobile and stationary states during their transport along axons
Journal of Neuroscience 27:507-516
PubMed | pdf | movies


Craciun G, Brown A & Friedman A (2005)
A dynamical system model of neurofilament transport in axons
Journal of Theoretical Biology 237:316-322
PubMed | pdf


Brown A, Wang L & Jung P (2005)
Stochastic simulation of neurofilament transport in axons: the “stop and go” hypothesis
Molecular Biology of the Cell 16:4243-4255
PubMed | pdf | movies


Yan Y & Brown A (2005)
Neurofilament polymer transport in axons
Journal of Neuroscience 25:7014-7021
PubMed | pdf | movie


Uchida A & Brown A (2004)
Arrival, reversal and departure of neurofilaments at the tips of growing axons
Molecular Biology of the Cell 15:4215-4225
PubMed | pdf | movies | MBC Paper of the Year Award


Brown A (2003)
Live-cell imaging of slow axonal transport
Methods in Cell Biology 71:305-323
PubMed | pdf


Brown A (2003)
Axonal transport of membranous and non-membranous cargoes: a unified perspective
Journal of Cell Biology 160:817-821
PubMed | pdf | Flash animation (requires Adobe Flash plugin)


Wang L & Brown A (2002)
Rapid movement of microtubules in axons
Current Biology 12:1496-1501
PubMed | pdf | movies


Wang L & Brown A (2001)
Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching
Molecular Biology of the Cell 12:3257-3267
PubMed | pdf | movies


Brown A (2000)
Slow axonal transport: stop and go traffic in the axon
Nature Reviews Molecular Cell Biology 1:153-156
PubMed | pdf


Wang L, Ho C-L, Sun D, Liem RKH & Brown A (2000)
Rapid movement of axonal neurofilaments interrupted by prolonged pauses
Nature Cell Biology 2:137-141
PubMed | pdf | movies | Featured in News and Views


Koehnle TJ & Brown A (1999)
Slow axonal transport of neurofilament protein in cultured neurons
Journal of Cell Biology 144:447-458
PubMed | pdf


Brown A (1998)
Contiguous phosphorylated and non-phosphorylated domains along axonal neurofilaments
Journal of Cell Science 111:455-467
PubMed | pdf


Brown A (1997)
Visualization of single neurofilaments by immunofluorescence microscopy of splayed axonal cytoskeletons
Cell Motility and the Cytoskeleton 38:133-145
PubMed | pdf


Baas PW & Brown A (1997)
Slow axonal transport: the polymer transport model
Trends in Cell Biology 7:380-384
PubMed | pdf | Commentary


Brown A & Lasek RJ (1995)
Polylysine cross-links axoplasmic neurofilaments into tight bundles
Cell Motility and the Cytoskeleton 31:9-21
PubMed | pdf


Black MM & Brown A (1993)
Sites of microtubule assembly in growing axons
In “Neuronal Cytoskeleton: Morphogenesis, Transport and Synaptic Transmission”, ed. N Hirokawa, Japan Scientific Societies Press, CRC Press, 171-182


Brown A & Lasek RJ (1993)
Neurofilaments move apart freely when released from the circumferential constraint of the axonal plasma membrane
Cell Motility and the Cytoskeleton 26:313-324
PubMed | pdf


Baas PW, Ahmad FJ, Pienkowski TP, Brown A & Black MM (1993)
Sites of microtubule stabilization for the axon
Journal of Neuroscience 13:2177-2185
PubMed | pdf


Brown A, Li Y, Slaughter T & Black MM (1993)
Composite microtubules of the axon: quantitative analysis of tyrosinated and acetylated tubulin along individual microtubules
Journal of Cell Science 104:339-342
PubMed | pdf


Brown A, Slaughter TS & Black MM (1992)
Newly assembled microtubules are concentrated in the proximal and distal regions of growing axons
Journal of Cell Biology 119:867-882
PubMed | pdf


Baas PW, Slaughter TS, Brown A & Black MM (1991)
Microtubule dynamics in axons and dendrites
Journal of Neuroscience Research 30:134-153
PubMed | pdf


Sahenk Z & Brown A (1991)
Weak-base amines inhibit the anterograde-to-retrograde conversion of axonally transported vesicles in nerve terminals
Journal of Neurocytology 20:365-375
PubMed | pdf


Brown A & Lasek RJ (1990)
The cytoskeleton of the squid giant axon
In “Squid as experimental animals”, ed. DL Gilbert, WJ Adelman Jr & JM Arnold, Plenum Publishing Corporation, NY, 235-302
pdf


Brown A & Eagles PAM (1986)
Squid neurofilaments: phosphorylation and Ca++-dependent proteolysis in situ
Biochemical Journal 239:191-197
PubMed | pdf